16th-century queens regnant

Mary, Queen of Scots (8 December 1542 – 8 February 1587), also known as Mary Stuart or Mary I of Scotland, was Queen of Scotland from 14 December 1542 until her forced abdication in 1567.

Elizabeth I (7 September 1533 – 24 March 1603) was Queen of England and Ireland from 17 November 1558 until her death in 1603. She was the last monarch of the House of Tudor.

Mary I (18 February 1516 – 17 November 1558), also known as Mary Tudor, and as "Bloody Mary" by her Protestant opponents, was Queen of England and Ireland from July 1553 and Queen of Spain from January 1556 until her death in 1558. She is best known for her vigorous attempt to reverse the English Reformation, which had begun during the reign of her father, Henry VIII. Her attempt to restore to the Church the property confiscated in the previous two reigns was largely thwarted by Parliament, but during her five-year reign, Mary had over 280 religious dissenters burned at the stake in the Marian persecutions.

Isabella I (Spanish: Isabel I; 22 April 1451 – 26 November 1504), also called Isabella the Catholic (Spanish: Isabel la Católica), was Queen of Castile from 1474 until her death in 1504. She was also Queen of Aragon from 1479 until her death as the wife of King Ferdinand II. Reigning together over a dynastically unified Spain, Isabella and Ferdinand are known as the Catholic Monarchs.

Lady Jane Grey (1536 or 1537 – 12 February 1554), later known as Lady Jane Dudley (after her marriage) and as the "Nine Days' Queen", was an English noblewoman who claimed the throne of England and Ireland from 10 July until 19 July 1553.

Jeanne d'Albret (Basque: Joana Albretekoa; Occitan: Joana de Labrit; 16 November 1528 – 9 June 1572), also known as Jeanne III, was Queen of Navarre from 1555 to 1572.

Joanna (6 November 1479 – 12 April 1555), historically known as Joanna the Mad (Spanish: Juana la Loca), was the nominal Queen of Castile from 1504 and Queen of Aragon from 1516 to her death in 1555. She was married by arrangement to Philip the Handsome, Archduke of Austria, of the House of Habsburg, on 20 October 1496. Following the deaths of her brother, John, Prince of Asturias, in 1497, her elder sister Isabella in 1498, and her nephew Miguel in 1500, Joanna became the heir presumptive to the crowns of Castile and Aragon. When her mother, Queen Isabella I of Castile, died in 1504, Joanna became Queen of Castile. Her father, King Ferdinand II of Aragon, proclaimed himself Governor and Administrator of Castile.

Catherine (Basque: Katalina, Occitan: Catarina; 1468 – 12 February 1517) was Queen of Navarre from 1483 until 1517. She was also Duchess of Gandia, Montblanc, and Peñafiel, Countess of Foix, Bigorre, and Ribagorza, and Viscountess of Béarn.

Malinxalchitl, meaning "beautiful flower of evil" (died 1524) was the Nahua queen regnant of Tzacoalco during the time of the Conquest of the Aztec Empire.

Aissa Koli also called Aisa Kili Ngirmaramma was a queen regnant in the Kanem–Bornu Empire in 1497–1504 or 1563–1570.